期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 300, 期 4, 页码 H1510-H1517出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00996.2010
关键词
skeletal muscle; blood flow; oxygen consumption; nitric oxide
资金
- Academy of Finland [108539, 214329]
- University of Turku
- Turku University Hospital
- Abo Academy
- Ministry of Education of the State of Finland [74/627/2006, 58/627/2007, 45/627/2008]
- Finnish Cultural Foundation
- Finnish Sport Research Foundation
- Novo Nordisk Foundation
- Danish Medical Research Council
- Academy of Finland (AKA) [214329, 108539, 214329, 108539] Funding Source: Academy of Finland (AKA)
Ilkka H, Bengt S, Jukka K, Sipil HT, Vesa O, Pirjo N, Juhani K, Kari K, Ylva H. Skeletal muscle blood flow and oxygen uptake at rest and during exercise in humans: a pet study with nitric oxide and cyclooxygenase inhibition. Am J Physiol Heart Circ Physiol 300: H1510-H1517, 2011. First published January 21, 2011; doi:10.1152/ajpheart.00996.2010.-The aim of the present study was to determine the effect of nitric oxide and prostanoids on microcirculation and oxygen uptake, specifically in the active skeletal muscle by use of positron emission tomography (PET). Healthy males performed three 5-min bouts of light knee-extensor exercise. Skeletal muscle blood flow and oxygen uptake were measured at rest and during the exercise using PET with H2O15 and O-15(2) during: 1) control conditions; 2) nitric oxide synthase (NOS) inhibition by arterial infusion of NG-monomethyl-L-arginine (L-NMMA), and 3) combined NOS and cyclooxygenase (COX) inhibition by arterial infusion of L-NMMA and indomethacin. At rest, inhibition of NOS alone and in combination with indomethacin reduced (P < 0.05) muscle blood flow. NOS inhibition increased (P < 0.05) limb oxygen extraction fraction (OEF) more than the reduction in muscle blood flow, resulting in an similar to 20% increase (P < 0.05) in resting muscle oxygen consumption. During exercise, muscle blood flow and oxygen uptake were not altered with NOS inhibition, whereas muscle OEF was increased (P < 0.05). NOS and COX inhibition reduced (P < 0.05) blood flow in working quadriceps femoris muscle by 13%, whereas muscle OEF and oxygen uptake were enhanced by 51 and 30%, respectively. In conclusion, by specifically measuring blood flow and oxygen uptake by the use of PET instead of whole limb measurements, the present study shows for the first time in humans that inhibition of NO formation enhances resting muscle oxygen uptake and that combined inhibition of NOS and COX during exercise increases muscle oxygen uptake.
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