期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 300, 期 5, 页码 H1822-H1828出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00766.2010
关键词
delayed afterdepolarization; triggered arrhythmia; calcium wave
资金
- National Heart, lung, and Blood Institute (NHLBI) [HL-090350, HL-084142, HL-100105]
Plummer RN, Cutler MJ, Wan X, Laurita KR. Spontaneous calcium oscillations during diastole in the whole heart: the influence of ryanodine reception function and gap junction coupling. Am J Physiol Heart Circ Physiol 300: H1822-H1828, 2011. First published March 4, 2011, doi:10.1152/ajpheart.00766.2010. - Triggered arrhythmias due to spontaneous cytoplasmic calcium oscillations occur in a variety of disease conditions; however, their cellular mechanisms in tissue are not clear. We hypothesize that spontaneous calcium oscillations in the whole heart are due to calcium release from the sarcoplasmic reticulum and are facilitated by calcium diffusion through gap junctions. Optical mapping of cytoplasmic calcium from Langendorff perfused guinea pig hearts (n = 10) was performed using oxygenated Tyrode's solution (in mM): 140 NaCl, 0.7 MgCl, 4.5 KCl, 5.5 dextrose, 5 HEPES, and 5.5 CaCl2, (pH 7.45, 34 degrees C). Rapid pacing was used to induce diastolic calcium oscillations. In all preparations, pacing-induced multicellular diastolic calcium oscillations (m-SCR) occurred across most of the mapping field, at all pacing rates tested. Ryanodine (1 mu M) eliminated all m-SCR activity. Low-dose caffeine (1 mM) increased m-SCR amplitude (+10.4 +/- 4.4%, P < 0.05) and decreased m-SCR time-to-peak (-17.4 +/- 6.7%, P < 0.05) and its temporal synchronization (i.e., range) across the mapping field (-26.9 +/- 17.1%, P < 0.05). Surprisingly, carbenoxolone increased the amplitude of m-SCR activity (+14.8 +/- 4.1%, P < 0.05) and decreased m-SCR time-to-peak (-11.3 +/- 9.6%, P < 0.01) and its synchronization (-37.0 +/- 19.1%, P < 0.05), similar to caffeine. In isolated myocytes, carbenoxolone (50 mu M) had no effect on the frequency of aftercontractions, suggesting the effect of cell-to-cell uncoupling on m-SCR activity is tissue specific. Therefore, in the whole heart, overt m-SCR activity caused by calcium release from the SR can be induced over a broad range of pacing rates. Enhanced ryanodine receptor open probability and, surprisingly, decreased cell-to-cell coupling increased the amplitude and temporal synchronization of spontaneous calcium release in tissue.
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