期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 296, 期 5, 页码 H1532-H1539出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00566.2008
关键词
smooth muscle; migration; adventitia
资金
- National Heart, Lung, and Blood Institute [HL-52490, HL-071574]
Fleenor BS, Bowles DK. Negligible contribution of coronary adventitial fibroblasts to neointimal formation following balloon angioplasty in swine. Am J Physiol Heart Circ Physiol 296: H1532-H1539, 2009. First published February 27, 2009; doi:10.1152/ajpheart.00566.2008.-Adventitial fibroblasts have previously been proposed to be a major constituent of the neointima following coronary balloon angioplasty. The present study utilized the bromodeoxyuridine (BrdU) pulse-chase technique to track adventitial fibroblast migration early after balloon injury in swine. BrdU (30 mg/kg), a marker of proliferating cells, was given intravenously 1 or 2 days after balloon angioplasty. For each time point, one animal was euthanized 24 h after injection to identify the location of the proliferating cells, while a second animal was euthanized 25 days after angioplasty to determine whether the proliferating cells migrated to form the neointima. Our results demonstrate that BrdU-positive cells were located primarily in the adventitia with all three time points 24 h after balloon angioplasty. Furthermore, when BrdU was injected on day 1 or 2 only 0.65 +/- 0.17% and 1.7 +/- 0.64%, respectively, of neointimal cells were BrdU positive on day 25. In conclusion, these results demonstrate a negligible contribution of coronary adventitial fibroblasts to neointima formation following coronary balloon angioplasty, supporting the concept that the neointima is primarily of smooth muscle cell origin.
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