期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 297, 期 1, 页码 H392-H399出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00164.2009
关键词
heme oxygenase-1; superoxide dismutase-2; peroxisome proliferator-activated receptor-gamma coactivator-1 alpha; nuclear respiratory factor-1; oxygen uptake
资金
- National Heart, Lung, and Blood Institute [1R01-HL-090679]
- Gas Enabled Medical Innovations (GEMI) Fund [393-3140]
Rhodes MA, Carraway MS, Piantadosi CA, Reynolds CM, Cherry AD, Wester TE, Natoli MJ, Massey EW, Moon RE, Suliman HB. Carbon monoxide, skeletal muscle oxidative stress, and mitochondrial biogenesis in humans. Am J Physiol Heart Circ Physiol 297: H392-H399, 2009. First published May 22, 2009; doi: 10.1152/ajpheart.00164.2009.-Given that the physiology of heme oxygenase-1 (HO-1) encompasses mitochondrial biogenesis, we tested the hypothesis that the HO-1 product, carbon monoxide (CO), activates mitochondrial biogenesis in skeletal muscle and enhances maximal oxygen uptake ((V) over dotO(2max)) in humans. In 10 healthy subjects, we biopsied the vastus lateralis and performed (V) over dotO(2max) tests followed by blinded randomization to air or CO breathing (1 h/day at 100 parts/million for 5 days), a contralateral muscle biopsy on day 5, and repeat (V) over dotO(2max) testing on day 8. Six independent subjects underwent CO breathing and two muscle biopsies without exercise testing. Molecular studies were performed by real-time RT-PCR, Western blot analysis, and immunochemistry. After (V) over dotO(2max) testing plus CO breathing, significant increases were found in mRNA levels for nuclear respiratory factor-1, peroxisome proliferator-activated receptor-gamma co-activator-1 alpha, mitochondrial transcription factor-A (Tfam), and DNA polymerase gamma (Pol gamma) with no change in mitochondrial DNA (mtDNA) copy number or (V) over dotO(2max). Levels of myosin heavy chain I and nuclear-encoded HO-1, superoxide dismutase-2, citrate synthase, mitofusin-1 and -2, and mitochondrial-encoded cytochrome oxidase subunit-I (COX-I) and ATPase-6 proteins increased significantly. None of these responses were reproduced by (V) over dotO(2max) testing alone, whereas CO alone increased Tfam and Pol gamma mRNA, and COX-I, ATPase-6, mitofusin-2, HO-1, and superoxide dismutase protein. These findings provide evidence linking the HO/CO response involved in mitochondrial biogenesis in rodents to skeletal muscle in humans through a set of responses involving regulation of the mtDNA transcriptosome and mitochondrial fusion proteins autonomously of changes in exercise capacity.
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