期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 295, 期 1, 页码 H154-H162出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.01327.2007
关键词
Brugada syndrome; acute ischemia; severe hyperkalemia
资金
- NHLBI NIH HHS [HL-47678, R01 HL047678] Funding Source: Medline
A greater depression of the action potential (AP) of the ventricular epicardium (Epi) versus endocardium (Endo) is readily observed in experimental models of acute ischemia and Brugada syndrome. Endo and Epi differences in transient outward K+ current and/or ATP-sensitive K+ channel current are believed to contribute to the differential response. The present study tested the hypothesis that the greater sensitivity of Epi is due in part to its functionally distinct early fast Na+ current (I-Na). APs were recorded from isolated Epi and Endo tissue slices and coronaryperfused wedge preparations before and after exposures to elevated extracellular K+ concentration ([K+](o); 6-12 mM). I-Na was recorded from Epi and Endo myocytes using whole cell patch-clamp techniques. In tissue slices, increasing [K+] o to 12 mM reduced V-max to 51.1 +/- 5.3% and 26.8 +/- 9.6% of control in Endo (n = 9) and Epi (n = 14), respectively (P < 0.05). In wedge preparations (n = 12), the increase in [K+](o) caused selective depression of Epi APs and transmural conduction slowing and block. I-Na density was not significantly different between Epi (n = 14) and Endo (n = 15) cells, but Epi cells displayed a more negative half-inactivation voltage [-83.6 +/- 0.1 and -75.5 +/- 0.3 mV for Epi (n = 16) and Endo (n = 16), respectively, P < 0.05]. Our data suggest that reduced I-Na availability in ventricular Epi may contribute to its greater sensitivity to electrical depression and thus may contribute to the R-ST segment changes observed under a variety of clinical conditions including acute myocardial ischemia, severe hyperkalemia, and Brugada syndrome.
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