期刊
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
卷 297, 期 5, 页码 G999-G1008出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpgi.00204.2009
关键词
inflammatory bowel diseases; wound healing; CXCR
资金
- Flight Attendant Medical Research Institute
- National Institute of Diabetes
- Digestive and Kidney Diseases [1KO1 DK083336, RO1 DK50894, RO1 DK69854, PO1 DK33506, RO1 DK072471, 1KO1 DK079015]
Im E, Choi YJ, Kim CH, Fiocchi C, Pothoulakis C, Rhee SH. The angiogenic effect of probiotic Bacillus polyfermenticus on human intestinal microvascular endothelial cells is mediated by IL-8. Am J Physiol Gastrointest Liver Physiol 297: G999-G1008, 2009. First published September 3, 2009; doi:10.1152/ajpgi.00204.2009.-Angiogenesis is required for wound healing and repair, but dysregulated angiogenesis is involved in gastrointestinal inflammation. Bacillus polyfermenticus (B. P.) is a probiotic bacterium clinically used for a variety of intestinal disorders in East Asia. Here we investigated the effect of B. P. on angiogenesis of human intestinal microvascular endothelial cells (HIMECs) and wound healing in intestinal mucosa. Exposure of HIMECs to the conditioned medium of B. P. cultures (B. P. CM) increased cell migration, permeability, and tube formation. Production of the proangiogenic cytokine IL-8 was increased by B. P. CM, and neutralizing antibodies against IL-8 or IL-8 receptor CXCR2 reduced tube formation as well as actin stress fiber formation. B. P. CM also increased NF-kappa B activation, and inhibitors of NF-kappa B suppressed B. P. CM-induced tube formation and IL-8 production. Furthermore, B. P. facilitated recovery of mice from colitis as shown by increased body weight and reduced rectal bleeding and histological severity. B. P. also increased angiogenesis and mouse IL-8 production in the mucosal layer. Collectively, these results show that B. P. increases angiogenesis of HIMECs in a NF-kappa B/IL-8/CXCR2-dependent manner. Moreover, B. P. promotes angiogenesis in the mucosa during recovery of mice from colitis, suggesting that this probiotic may be clinically used to facilitate intestinal wound healing.
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