期刊
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
卷 304, 期 5, 页码 E516-E523出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpendo.00435.2012
关键词
intrauterine growth restriction; pancreas; glucose; oxygen; norepinephrine
资金
- National Institutes of Health (NIH) [RO1-HD-42815, R01-DK-084842, R01-DK-088139, K08-HD-060688, T32 HL-7249, HD-07186]
- NIH-Colorado Clinical Nutrition Research Unit [P30-DK-048520-11]
Reductions in insulin concentrations and beta-cell mass precede growth restriction in sheep fetuses with placental insufficiency. Am J Physiol Endocrinol Metab 304: E516-E523, 2013. First published December 31, 2012; doi:10.1152/ajpendo.00435.2012.-In pregnancy complicated by placental insufficiency (PI) and intrauterine growth restriction (IUGR), the fetus near term has reduced basal and glucose-stimulated insulin concentrations and reduced beta-cell mass. To determine whether suppression of insulin concentrations and beta-cell mass precedes reductions in fetal weight, which would implicate insulin deficiency as a cause of subsequent IUGR, we measured basal and glucose-stimulated insulin concentrations and pancreatic histology at 0.7 gestation in PI fetuses. Placental weights in the PI pregnancies were 40% lower than controls (265 +/- 26 vs. 442 +/- 41 g, P < 0.05), but fetal weights were not different. At basal conditions blood oxygen content, plasma glucose concentrations, and plasma insulin concentrations were lower in PI fetuses compared with controls (2.5 +/- 0.3 vs. 3.5 +/- 0.3 mmol/l oxygen, P < 0.05; 1.11 +/- 0.09 vs. 1.44 +/- 0.12 mmol/l glucose; 0.12 +/- 0.01 vs. 0.27 +/- 0.02 ng/ml insulin; P < 0.05). During a steady-state hyperglycemic clamp ( 2.5 +/- 0.1 mmol/l), glucose-stimulated insulin concentrations were lower in PI fetuses than controls (0.28 +/- 0.02 vs. 0.55 +/- 0.04 ng/ml; P < 0.01). Plasma norepinephrine concentrations were 3.3-fold higher (P < 0.05) in PI fetuses (635 +/- 104 vs. 191 +/- 91 pg/ml). Histological examination revealed less insulin area and lower beta-cell mass and rates of mitosis. The pancreatic parenchyma was also less dense (P < 0.01) in PI fetuses, but no differences were found for pancreatic progenitor cells or other endocrine cell types. These findings show that hypoglycemia, hypoxemia, and hypercatecholaminemia are present and potentially contribute to lower insulin concentrations and beta-cell mass due to slower proliferation rates in early third-trimester PI fetuses before discernible reductions in fetal weight.
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