期刊
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
卷 304, 期 8, 页码 E895-E907出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpendo.00579.2012
关键词
protein kinetics; protein degradation; fractional breakdown rate; deuterated water
资金
- H:S (Region Hovedstaden)
- Faculty of Health Sciences, University of Copenhagen
- Anti Doping Denmark
- Danish Council for Independent Research [09-073587]
- National Institute of Diabetes and Digestive and Kidney Diseases [R01-DK-038429]
Holm L, O'Rourke B, Ebenstein D, Toth MJ, Bechshoeft R, Holstein-Rathlou N, Kjaer M, Matthews DE. Determination of steady-state protein breakdown rate in vivo by the disappearance of protein-bound tracer-labeled amino acids: a method applicable in humans. Am J Physiol Endocrinol Metab 304: E895-E907, 2013. First published February 19, 2013; doi:10.1152/ajpendo.00579.2012.-A method to determine the rate of protein breakdown in individual proteins was developed and tested in rats and confirmed in humans, using administration of deuterium oxide and incorporation of the deuterium into alanine that was subsequently incorporated into body proteins. Measurement of the fractional breakdown rate of proteins was determined from the rate of disappearance of deuterated alanine from the proteins. The rate of disappearance of deuterated alanine from the proteins was calculated using an exponential decay, giving the fractional breakdown rate (FBR) of the proteins. The applicability of this protein-specific FBR approach is suitable for human in vivo experimentation. The labeling period of deuterium oxide administration is dependent on the turnover rate of the protein of interest.
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