期刊
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
卷 304, 期 3, 页码 C248-C256出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00039.2012
关键词
pericam; gene transfer; calcium oscillations; adenovirus; sorcin overexpression; apoptosis
资金
- National Heart, Lung, and Blood Institute [HL-66917, HL-52496, 3R01 HL-066917-10S1]
- American Heart Association, Western Affiliates [0150735]
Suarez J, McDonough PM, Scott BT, Suarez-Ramirez A, Wang H, Fricovsky ES, Dillmann WH. Sorcin modulates mitochondrial Ca2+ handling and reduces apoptosis in neonatal rat cardiac myocytes. Am J Physiol Cell Physiol 304: C248-C256, 2013. First published November 14, 2012; doi: 10.1152/ajpcell.00039.2012.Sorcin localizes in cellular membranes and has been demonstrated to modulate cytosolic Ca2+ handling in cardiac myocytes. Sorcin also localizes in mitochondria; however, the effect of sorcin on mitochondrial Ca2+ handling is unknown. Using mitochondrial pericam, we measured mitochondrial Ca2+ concentration and fluxes in intact neonatal cardiac myocytes overexpressing sorcin. Our results showed that sorcin increases basal and caffeine-stimulated mitochondrial Ca2+ concentration. This effect was associated with faster Ca2+ uptake and release. The effect of sorcin was specific for mitochondria, since similar results were obtained with digitonin-permeabilized cells, where cytosolic Ca2+ flux was disrupted. Furthermore, mitochondria of cardiac myocytes in which sorcin was overexpressed were more Ca2+-tolerant. Experiments analyzing apoptotic signaling demonstrated that sorcin prevented 2-deoxyglucose-induced cytochrome c release. Furthermore, sorcin prevented hyperglycemia-induced cytochrome c release and caspase activation. In contrast, antisense sorcin induced caspase-3 activation. Thus, sorcin antiapoptotic properties may be due to modulation of mitochondrial Ca2+ handling in cardiac myocytes.
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