期刊
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
卷 296, 期 6, 页码 C1364-C1372出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpcell.00014.2009
关键词
inactivating A-type outward K+ current; B-1/B-2 receptor; adenosine 3 ',5 '-cyclic monophosphate/protein kinase A
资金
- Committee of Science and Technology, Shanghai [04DZ19901]
- National Basic Research Program of China [2007CB512303]
- Shanghai Leading Academic Discipline Project [B111]
- National Talent Training Fund in Basic Research of China [J0630643]
Zhang M, Fei XW, He YL, Yang G, Mei YA. Bradykinin inhibits the transient outward K+ current in mouse Schwann cells via the cAMP/PKA pathway. Am J Physiol Cell Physiol 296: C1364-C1372, 2009. First published April 1, 2009; doi:10.1152/ajpcell.00014.2009. Bradykinin (BK) is an endogenous peptide with diverse biological actions and is considered to be an important mediator of the inflammatory response in both the peripheral and the central nervous systems. BK has attracted recent interest as a potential mediator of K+ conductance, Cl- channels, and Ca2+-activated K+ channels. However, few reports have associated BK with the voltage-gated K+ current. In this study, we demonstrated that BK suppressed the transient outward potassium current (I-A) in mouse Schwann cells using whole cell recording techniques. At a concentration of 0.1 mu M to 5 mu M, BK reversibly inhibited I-A in a dose-dependent manner with the modulation of steady-state activation and inactivation properties. The effect of BK on I-A current was abolished after preincubation with a B-2 receptor antagonist but could not be eliminated by B-1 receptor antagonist. Intracellular application of GTP-gamma S induced an irreversible decrease in I-A, and the inhibition of G(s) using NF449 provoked a gradual augmentation in I-A and eliminated the BK-induced effect on I-A, while the G(i)/(o) antagonist NF023 did not. The application of forskolin or dibutyryl-cAMP mimicked the inhibitory effect of BK on I-A and abolished the BK-induced effect on I-A. H-89, an inhibitor of PKA, augmented I-A amplitude and completely eliminated the BK-induced inhibitory effect on I-A. In contrast, activation of PKC by PMA augmented I-A amplitude. A cAMP assay revealed that BK significantly increased intracellular cAMP level. It is therefore concluded that BK inhibits the I-A current in Schwann cells by cAMP/PKA-dependent pathways via activation of the B-2 receptor.
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