期刊
TRANSGENIC RESEARCH
卷 17, 期 3, 页码 337-344出版社
SPRINGER
DOI: 10.1007/s11248-007-9106-y
关键词
transformation; marker-free; site-specific recombination; RMCE; R/RS; Agrobacterium
Recently, site-specific recombination methods in plants have been developed to delete selection markers to produce marker-free transgenic plants or to integrate the transgene into a pre-determined genomic location to produce site-specific transgenic plants. However, these methods have been developed independently, and although the strategies of producing marker-free site-specific integration plants have been discussed, the concept has not been demonstrated. In the present study, we combined two approaches to site-specific recombination and demonstrated the concepts for removing the marker after site-specific integration for producing marker-free site-specific transgenic plants.
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