期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 182, 期 3, 页码 852-865出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2012.11.035
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资金
- Canadian Institute for Health Research (CIHR)
- Crohn's and Colitis Foundation of Canada (CCFC)
- Alberta Inflammatory Bowel Disease Consortium
- Alberta Innovates Health Solutions Studentships
- University of Calgary scholarships
Human mucin-2 (MUC-2) is the first line of innate host defense in preventing pathogen-induced epithelial injury. Entamoeba histolytica (Eh) colonizes the mucus layer by binding of the parasite's surface galactose lectin to galactose and N-acetyl-D-galactosamine residues on colonic MUC-2, preventing parasite contact-dependent cytolysis of epithelial cells. We quantified early innate responses to Eh in wild-type and MUC-2-deficient mice (Muc2(-/-)) using closed colonic loops. Eh infection in wildtype but not Muc2(-/-) mice induced a time-dependent increase in H-3-labeled mucin and nonmucin glycoprotein secretions. Immunohistochemical staining revealed intense MUC-2 secretion, which formed a thick, protective mucus plug overlying the surface epithelium, entrapping Eh. In Muc2(-/-) mice, Eh induced a pronounced time-dependent secretory exudate with increased gross pathology scores and serum albumin leakage. Colonic pathology, secretory responses, and increased proinflammatory cytokine secretions of TNF-alpha, IFN-gamma, and IL-13 correlated with altered expression of the tightjunction proteins claudin-2, occludin, and Z0-1. We identified the putative Eh virulence factor that elicits the proinflammatory responses and alters tight junction permeability as Eh cysteine protease A5 (EhCP-A5). The present findings demonstrate that colonic mucins confer both luminal and epithelial barrier functions and that, in the absence of MUC-2, mice are more susceptible to Eh-induced secretory and proinflammatory responses mediated by EhCP-A5. (Am 3 Pathol 2013, 182: 852-865; http:// dx.doi.org/10.1016/jajpath.2012.11.035)
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