期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 179, 期 4, 页码 1733-1745出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2011.06.041
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资金
- National Institutes of Health [HL090669, CA122086, CA128660, HL0894932, HL68628]
- Canadian Institutes of Health Research [MOP 84254]
- Harold and Mary Zirin Chair in Pulmonary Biology at National Jewish Health
- Schulich School of Medicine and Dentistry
Idiopathic pulmonary fibrosis (IPF) may be triggered by epithelial injury that results in aberrant production of growth factors, cytokines, and proteinases, leading to proliferation of myofibroblasts, excess deposition of collagen, and destruction of the lung architecture. The precise mechanisms and key signaling mediators responsible for this aberrant repair process remain unclear. We assessed the importance of matrix metalloproteinase-3 (MMP-3) in the pathogenesis of IPF through i) determination of MMP-3 expression in patients with IPF, in vivo experiments examining the relevance of MMP-3 in experimental models of fibrosis, and in vitro experiments to elucidate possible mechanisms of action. Gene expression analysis, quantitative RT-PCR, and Western blot analysis of explanted human lungs revealed enhanced expression of MMP-3 in IPF, compared with control Transient adenoviral vector-mediated expression of recombinant MMP-3 in rat lung resulted in accumulation of myofibroblasts and pulmonary fibrosis. Conversely, MMP-3-null mice were protected against bleomycin-induced pulmonary fibrosis. In vitro treatment of cultured lung epithelial cells with purified MMP-3 resulted in activation of the beta-catenin signaling pathway, via cleavage of E-cadherin, and induction of epithelial-mesenchymal transition. These processes were inhibited in bleomycin-treated MMP-3-null mice, as assessed by cytosolic translocation of beta-catenin and cyclin D1 expression. These observations support a novel role for MMP-3 in the pathogenesis of IPF, through activation of beta-catenin signaling and induction of epithelial-mesenchymal transition. (Am J Pathol 2011, 179:1733-1745; DOI: 10.1016/j.ajpath.2011.06.041)
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