期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 178, 期 2, 页码 828-837出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ajpath.2010.10.026
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- SUS Malmo ALF
- Swedish Cancer Society
- Swedish Research Council
- Crafoord Foundation
- Gunnar Nilsson's Cancer Foundation
- National Association for Kidney Diseases
- Malmo University Hospital
- Ollie and Elof Ericsson's Foundation
- Swedish Society for Medical Research
The tubules of the kidney display a remarkable capacity for self-renewal on damage. Whether this regeneration is mediated by dedifferentiating surviving cells or, as recently suggested, by stern cells has not been unequivocally settled. Herein, we demonstrate that aldehyde dehydrogenase (ALDH) activity may be used for isolation of cells with progenitor characteristics from adult human renal cortical tissue. Gene expression profiling of the isolated ALDH(high) and ALDH(low) cell fractions followed by immunohistochemical interrogation of renal tissues enabled us to delineate a tentative progenitor cell population scattered through the proximal tubules (PTs). These cells expressed CD24 and 03133, previously described markers for renal progenitors of Bowman's capsule. Furthermore, we show that the PT cells, and the glomerular progenitors, are positive for KRT7, KRT19, BCL2, and vimentin. In addition, tubular epithelium regenerating on acute tubular necrosis displayed long stretches of CD133(+)/VIM+ cells, further substantiating that these cells may represent a progenitor cell population. Furthermore, a potential association of these progenitor cells with papillary renal cell carcinoma was discovered. Taken together, our data demonstrate the presence of a previously unappreciated subset of the PT cells that may be endowed with a more robust phenotype, allowing increased resistance to acute renal injury, enabling rapid repopulation of the tubules. (Am J Pathol 2011, 178:828-837; DOI: 10.1016/j.ajpath.2010.10.026)
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