期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 177, 期 1, 页码 493-500出版社
ELSEVIER SCIENCE INC
DOI: 10.2353/ajpath.2010.090480
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资金
- Swedish Heart and Lung Foundation
- Swedish Research Council
- Swedish Society of Medicine
- Swedish Society for Medical Research
- Osterman Fund
- Tore Nilson Foundation
- Lars Hierta Memorial Fund
- AFA Health Fund
- AstraZeneca
- Karolinska Institute
- Deutsche Forschungsgemeinschaft [SO876/1-1]
Inflammation and activation of immune cells are key mechanisms in the development of atherosclerosis. Previous data indicate important roles for monocytes and T-lymphocytes in lesions. However, recent data suggest that neutrophils also may be of importance in atherogenesis. Here, we use apolipoprotein E (ApoE)deficient mice with fluorescent neutrophils and monocytes (ApoE(-/-)/Lys(EGFP)/(EGFP) mice) to specifically study neutrophil presence and recruitment in atherosclerotic lesions. We show by flow cytometry and confocal microscopy that neutrophils make up for 1.8% of CD45(+) leukocytes in the aortic wall of ApoE(-/-)/Lys(EGFP)/(EGFP) mice and that their contribution relative to monocyte/macrophages within lesions is approximately 1:3. However, neutrophils accumulate at sites of monocyte high density, preferentially in shoulder regions of lesions, and may even outnumber monocyte/macrophages in these areas. Furthermore, intravital microscopy established that a majority of leukocytes interacting with endothelium on lesion shoulders are neutrophils, suggesting a significant recruitment of these cells to plaque. These data demonstrate neutrophilic granulocytes as a major cellular component of atherosclerotic lesions in ApoE(-/-) mice and call for further study on the roles of these cells in atherogenesis. (Am J Pathol 2010, 177:493-500; DOI: 10.2353/ajpath.2010.090480)
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