期刊
AMERICAN JOURNAL OF PATHOLOGY
卷 175, 期 4, 页码 1733-1745出版社
ELSEVIER SCIENCE INC
DOI: 10.2353/ajpath.2009.090133
关键词
-
类别
资金
- Stem Cell Research Center of the 21st Century Frontier Research Program [SC-5120]
- Ministry of Education, Science and Technology, Korea [R2009-0079390, R31-2008-000-10071-0]
- National Research Foundation of Korea [2009-0079390] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Lymphatic vessels in the diaphragm are essential for draining peritoneal fluid, but little is known about their pathological changes during inflammation. Here we characterized diaphragmatic lymphatic vessels in a peritonitis model generated by daily i.p. administration of lipopolysaccharide (LPS) in mice. Intraperitoneal LPS increased lymphatic density, branching, sprouts, connections, and network formation in the diaphragm in time- and dose-dependent manners. These changes were reversible on discontinuation of LPS administration. The LPS-induced lymphatic density and remodeling occur mainly through proliferation of lymphatic endothelial cells. CD11b(+) macrophages were massively accumulated and closely associated with the lymphatic vessels changed by i.p. LPS. Both RT-PCR assays and experiments with vascular endothelial growth factor-C/D blockade and macrophage-depletion indicated that the CD11b(+) macrophage-derived lymphangiogenic factors vascular endothelial growth factor-C/D could he major mediators of LPS-induced lymphangiogenesis and lymphatic remodeling through paracrine activity. Functional assays with India Ink and fluorescein isothiocyanate-microspheres indicated that impaired peritoneal fluid drainage in diaphragm of LPS-induced peritonitis mice was due to inflammatory fibrosis and massive attachment of CD11b(+) macrophages on the peritoneal side of the diaphragmatic lymphatic vessels.. These findings reveal that CD11b(+) macrophages play an important role in i.p. LPS-induced aberrant lymphangiogenesis and lymphatic dysfunction in the diaphragm. (Am J Pathol 2009, 175:1733-1733; DOI: 10.2353/ajpath.2009.090133)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据