期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 211, 期 -, 页码 44-50出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2015.06.025
关键词
Antifungal; Antioxidant; Biopreservation; Cytotoxicity; 2,4-Di-tert-butyl phenol; Lactic acid bacteria
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
- CSIR grant under XIIth Five Year Plan Network Project, FUNHEALTH [CSC 0133]
The volatile organic compound 2,4-di-tert-butyl phenol (2,4 DTBP) was purified from the cell free supernatant of a newly isolated Lactococcus sp. by solvent extraction and chromatographic techniques. Molecular characterization of the compound by ESI-MS, H-1 NMR and FTIR analysis revealed the structure, C14H22O. Fungicidal activity was demonstrated against Aspergillus niger, Fusarium oxysporum and Penicillium chrysogenum by disc diffusion assay. Among the cell lines tested for cytotoxicity of this compound (normal cell line H9c2 and cancer cell lines HeLa and MCF-7), a remarkable cytotoxicity against HeLa cells with an IC50 value of 10 mu g/mL was shown. A biocontrol experiment with 2,4 DTBP supplemented fraction prevented growth of the abovementioned fungi on wheat grains. The study further strengthens the case for development of biopreservatives and dietary antioxidants from lactic acid bacteria for food applications. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据