期刊
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
卷 202, 期 5, 页码 -出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.ajog.2010.01.057
关键词
cysteine-rich protein 61; miR-155; placenta; preeclampsia
资金
- Chinese National Natural Science Foundation [30872775]
- Science and Technology Ministry of China [2010CB945104]
- Health Department of Jiangsu Province [LJ200628, XK200709]
- Jiangsu Provincial Government, China
OBJECTIVE: The aim of this study was to characterize the molecular mechanism of preeclampsia (PE) development through miR-155. STUDY DESIGN: PE and normal placentas were used to measure miR-155 and cysteine-rich protein 61 (CYR61) expression. CYR61 3' untranslated region was validated as the target of miR-155 using in vitro transfections. miR-155 and CYR61 expression levels were assessed by real-time reverse transcription polymerase chain reaction or Western blot. RESULTS: An inverse correlation was found between miR-155 and CYR61 expression levels, with miR-155 up-regulated and CYR61 down-regulated in PE tissues. Luciferase assays and CYR61 transfection assays experimentally validated that miR-155 efficiently targets the 3' untranslated region of CYR61. CONCLUSION: This study reported for the first time that overexpression of miR-155 contributes to PE development by targeting and down-regulating angiogenic regulating factor CYR61, leading to pathological alterations. This finding not only characterizes a new mechanism for the disease but also provides a potential therapeutic target.
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