期刊
AMERICAN JOURNAL OF NEPHROLOGY
卷 32, 期 3, 页码 194-200出版社
KARGER
DOI: 10.1159/000316528
关键词
Transient receptor potential vanilloid type 1; Afferent renal nerve activity; Dahl rats; Salt sensitivity; Substance P; Calcitonin gene-related peptide
资金
- National Institutes of Health [HL-57853, HL-73287, DK67620]
- Michigan Economic Development Corporation
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL073287, R01HL057853] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK067620] Funding Source: NIH RePORTER
Objective: To test the hypothesis that transient receptor potential vanilloid type 1 channel (TRPV1)-mediated increases in afferent renal nerve activity (ARNA) and release of substance P (SP) and calcitonin gene-related peptide (CGRP) from the renal pelvis are suppressed in Dahl salt-sensitive (DS), but not -resistant (DR), rats fed a high-salt (HS) diet. Methods and Results: Male DS and DR rats were given a HS or low-salt (LS) diet for 3 weeks. Perfusion of capsaicin (CAP, 10(-6) M), a selective TRPV1 agonist, into the left renal pelvis increased ipsilateral ARNA in all groups, but with a smaller magnitude in DS-HS compared to other groups. CAP increased contralateral urine flow in all groups except DS-HS rats. CAP-induced release of SP and CGRP from the renal pelvis was less in DS-HS compared to other groups. Western blot showed that TRPV1 expression in the kidney decreased while expression of neurokinin 1 receptors increased in DS-HS compared to other groups. Conclusion: TRPV1-mediated increases in ARNA and release of SP and CGRP in the renal pelvis are impaired in DS rats fed a HS diet, which can likely be attributed to suppressed TRPV1 expression in the kidney and contributes to increased salt sensitivity. Copyright (C) 2010 S. Karger AG, Basel
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