Preparation of magnesia-zirconia based mimetic biomembrane stationary phase and its applications in evaluating drug-membrane interactions

A novel mimetic biomembrane chromatographic stationary phase of magnesiazirconia composite matrix was prepared based on the Lewis acid-base interaction between the phosphonate group of phosphatidylcholine residue and the Lewis acid sites of magnesia-zirconia composite. The infrared absorption spectrum and X-ray photoelectron spectrum of the stationary phase illustrated that the magnesia-zirconia composite was successfully modified with phosphatidylcholine. The interactions between the membrane and the drugs were evaluated. It is observed that the log K-mbm values have good relationships with the log P-app, and the linear slope is 1. 049 which is near unity. Moreover, on the basis of the thermodynamics derivation, the difference in standard free energies (Delta(Delta G degrees)) is introduced to describe the drug-membrane interaction. The results show that the log K-mbm and Delta (Delta G) value provide key information on the transport properties of the drugs. The establishment of this chromatographic model may be a new way for the evaluation of the drug-membrane interactions.