Effect of the Polymer Architecture on the Structural and Biophysical Properties of PEG-PLA Nanoparticles

Polymers made of poly,(ethylene glycol) chains grafted to poly(lactic add) chains (PEG-g-PLA) were used to produce stealth drug nanocarriers. A library of comblike PEG-g-PLA polymers with different PEG grafting densities was prepared in order to obtain nanocarriers with dense PEG brushes at their surface, stability in suspension, and resistance to protein adsorption. The structural properties of nanoparticles(NPs) produced from these polymers by a surfactant-free method were assessed by dynamic light scattering, zeta potential, and transmission electron microscopy and found to be controlled by the amount of PEG present in the polymers. Are critical transition from a solid NP structure to a soft particle with either a micellelike or a polymer nanoaggregate structure was observed when the PEG content was between 15 and 25% w/w. This structural transition was found to have a profound impact on the size of the NPs, their surface charge, their stability in suspension in the presence of salts, and the binding of proteins to the surface of the NPs. The arrangement of the PEG-g-PLA Chains at the surface of the NPs was investigated by H-1 NMR and X-ray photoelectron spectroscopy (XPS). NMR results confirmed that the PEG chains were mostly segregated at the NP Surface. Moreover, XPS and quantitative NMR,allowed quantification of the PEG chain Coverage density at the surface of the solid NPs. Concordance of the results between the two methods was found to he remarkable., Physical chemical properties of the NPs such as resistance to aggregation in a saline environment as well as antifouling efficacy were related to the PEG surface density and ultimately to the polymer architecture. Resistance to protein adsorption was assessed by isothermal titration calorimetry using lysozyme. The results indicate a correlation between the PEG surface coverage and level of protein interactions:The results obtained lead us to propose such