期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 142, 期 1, 页码 29-34出版社
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCP8QMOY5GLRSFP
关键词
Membranous nephropathy; Phospholipase A2 receptor 1; Nephrotic syndrome; ELISA; Sensitivity and specificity
类别
资金
- National Institutes of Health, Bethesda, MD [DK090029, DK097053]
- NephCure Foundation, Berwyn, PA
Objectives: Autoantibodies against the M-type phospholipase A2 receptor 1 (anti-PLA2R1) have been demonstrated to be very specific for idiopathic membranous nephropathy (MN). We studied a novel enzyme-linked immunosorbent assay (ELISA) and compared results with results obtained using an indirect immunofluorescence (IF) and a Western blotting test (WB). Methods: One-hundred nine patients with idiopathic MN were recruited between November 1979 and March 2011. The control cohort comprised serum samples from patients with secondary MN (n = 16) and nephrotic controls (n = 17). The presence of anti-PLA2R1 in serum samples obtained at the time of renal biopsy was determined using ELISA, IIF, and WB. Results: With similar specificity (>= 97%), sensitivity varied from 68% (IIF) to 72% (ELISA, WB). Remarkably, patients who were seronegative for anti-PLA2R1 more often entered spontaneous remission (P = .038), whereas seropositive patients were more frequently treated with immunosuppressive agents (P < .001). Conclusions: ELISA performs excellently in differentiating idiopathic from secondary MN. Furthermore, ELISA shared high agreement with WB and IIF.
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