期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 140, 期 2, 页码 155-164出版社
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCPCLHZR5KUHUBM
关键词
Flow cytometry; GCSF-R; CD114; Myelodysplastic syndrome; Chronic myelogenous leukemia
类别
Objectives: To characterize granulocyte colony-stimulating factor receptor (CD114) expression in normal (n = 20), myelodysplastic (n = 34), and chronic myelogenous leukemia (CML; n = 5) bone marrow by flow cytometry. Methods: Clinical bone marrow samples were analyzed using CD33/CD114/CD34/CD117/CD45. CD114 density (mean fluorescence intensity) and cellular distribution were evaluated on early blasts (CD33-), late blasts (CD33+), promyelocytes, and granulocytes. Results: Normal CD114 acquisition occurred on early blasts, peaked on promyelocytes, and decreased on granulocytes. Forty percent of CD34+ blasts expressed CD114 and one-third were early blasts. In myelodysplastic syndromes, altered CD114 distribution was more informative than density changes. In CML, CD114 density was significantly decreased on early blasts and expression was essentially limited to late blasts. We observed a specific blast dysmatirration pattern in CML involving CD33, CD34, and CD114 that was 83% sensitive and 100% specific in initial diagnosis. Conclusions: CD114 provides useful additional detail in phenotypic assessment of hematopoietic precursor
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