期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 138, 期 4, 页码 590-593出版社
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCP6HZI5DDQTCRM
关键词
Plasma cell dyscrasia; Trephine bone marrow biopsy; Amyloid; Congo red; Fluorescence microscopy; Polarized microscopy; Immunohistochemistry
类别
The classic gold standard for detecting amyloid deposits is Congo red stained bright field and polarized microscopy (CRPM). A prior study showed that Congo red fluorescence (CRF) microscopy had increased sensitivity compared with traditional CRPM when analyzing fat pad specimens. The purpose of the current study was to determine the sensitivity of CRF for evaluating Congo red stained bone marrow biopsy specimens, and to compare these results with those of CRPM We compared the CRPM and the CRF analyses of 33 trephine bone marrow biopsy specimens with clinical or morphologic suspicion of amyloid deposits. These results were verified against immunohistochemical staining with anti amyloid P antibody. CRF achieved 100% sensitivity, and CRPM achieved 75% sensitivity. Both groups showed 100% specificity compared with amyloid P immunohistochemical staining. The results show that CRF is a sensitive method to analyze trephine bone marrow biopsy specimens for amyloid deposits.
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