期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 134, 期 5, 页码 799-806出版社
OXFORD UNIV PRESS INC
DOI: 10.1309/AJCPTFDSE2V3LCZP
关键词
Hepatocellular carcinoma; Insulin-like growth factor II (IGF-II); IGF-II mRNA; Gene amplification; Metastasis; Peripheral blood mononuclear cells
类别
资金
- Natural Science Foundation of Jiangsu Province [BK2008187]
- Natural Science Project of Nantong University [09ZY013]
The prognosis of hepatocellular carcinoma (HCC) remains dismal. Insulin-like growth. factor II (IGF-II), a fetal growth factor, is highly expressed during HCC development. We examined serum IGF-II levels and circulating IGF-II messenger RNA (mRNA) expression and analyzed the clinicopathologic characteristics in patients with liver diseases. The higher IGF-II level in the serum of patients with HCC could be correlated with hepatitis B virus infection but not with patient sex, age, tumor size, or a-fetoprotein (AFP) level. Total RNAs were extracted from liver tissues or peripheral blood mononuclear cells, and IGF-II complementary DNA (cDNA) and AFP cDNA were synthesized through random primers and reverse transcriptase; gene fragments were amplified by nested polymerase chain reaction and confirmed by sequencing. The incidence of the hepatic IGF-II gene was 100% in HCC, 54.3% in paracancerous tissues, and none in noncancerous tissues. The incidence rates for circulating IGF-II and APP genes were 34.3% and 52.7%, respectively, and for both, 61.6% in patients with HCC. They were 100% in cases with extrahepatic metastasis. The IGF-II abnormality associates with HCC, and circulating IGF-II and IGF-II mRNA are useful molecular markers for HCC differential diagnosis and hematogenous metastasis.
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