期刊
AMERICAN JOURNAL OF CLINICAL NUTRITION
卷 98, 期 2, 页码 413-422出版社
OXFORD UNIV PRESS
DOI: 10.3945/ajcn.113.060996
关键词
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资金
- Canadian Institutes of Health Research [MOP 77596]
- McGill University Health Centre Research Institute Fellowship
- Fonds de Recherche du Quebec-Sante
Background: beta,beta-Carotene 15,15'-monooxygenase (BCMO1) converts beta-carotene to retinaldehyde. Increased beta-carotene consumption is linked to antitumor effects. Retinoic acid reduces the invasiveness in cancer, through inhibition of matrix metalloproteinases (MMPs). In our studies of a mouse model that develops intestinal tumors after low dietary folate, we found reduced BCMO1 expression in normal preneoplastic intestine of folate-deficient tumor-prone mice. Objective: Our goal was to determine whether BCMO1 expression could influence transformation potential in human colorectal carcinoma cells, by examining the effect of BCMO1 modulation on cellular migration and invasion, and on expression of MMPs. Design: LoVo colon carcinoma cells were transfected with BCMO1 small interfering RNA (siRNA) or scrambled siRNA. Migration and invasion were measured, and the expression of BCMO1,MMP7, and MMP28 was assessed by quantitative reverse-transcriptase polymerase chain reaction. These variables were also measured after treatment of cells with retinoic acid, 5-aza-2'-deoxycytidine, folate-depleted/high-methionine medium, and beta-carotene. Results: Retinoic acid decreased the migration, invasion, and expression of MMP28 mRNA. Transfection of cells with BCMO1 siRNA inhibited BCMO1 expression, enhanced migration and invasion, and increased expression of MMP7 and MMP28. 5-Aza-2'-deoxycytidine decreased, whereas folate-depleted/high-methionine medium increased invasiveness. beta-Carotene increased BCMO1 expression and reduced invasiveness with a decrease in expression of MMP7 and MMP28. Conclusions: Inhibition of BCMO1 expression is associated with increased invasiveness of colon cancer cells and increased expression of MMP7 and MMP28. beta-Carotene can upregulate BCMO1 and reverse these effects. These novel associations suggest a critical role for BCMO1 in cancer and provide a mechanism for the proposed antitumor effects of beta-carotene.
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