Effect of Curculigo orchioides on Reflux Esophagitis by Suppressing Proinflammatory Cytokines

This study was performed to investigate effects of Curculigo orchioides rhizome (curculiginis rhizome) on acute reflux esophigitis (RE) in rats that are induced by pylorus and forestomach ligation operation. Proinflammatory cytokine, as well as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta and IL-6 were all assayed and the expression of TNF-alpha and COX2 analyzed by RT-PCR. The esophagic tissue damage of reflux esophagitis rat was increased compared to that of normal intact group. However, the esophagic damage percentage from the extract of curculiginis rhizoma (ECR) 600 mg/kg and ECR 300 mg/kg were significantly lower than that of the RE control group. Administration of alpha-tocopherol (30 mg/kg) and ECR (600 mg/kg, 300 mg/kg, and 150 mg/kg) had a significant effect on the gastric acid pH in rats with induced reflux esophagitis (p < 0: 05). The treatment with ECR significantly reduced the production of cytokines TNF-alpha, IL-1 beta and IL-6 levels compared to the model group (p < 0.05). The expression of TNF-alpha and COX2 in the intact esophageal mucosa was low while those of the RE control group were significantly higher due to an inflammatory reaction in the esophagus. Compare to the model group, treatment with alpha-tocopherol or ECR significantly inhibited the expression levels of COX2 and TNF-alpha in a dose-dependent manner. These results suggest that anti-inflammatory and protective effects of ECR could attenuate the severity of reflux esophagitis and prevent esophageal mucosal damage.