期刊
AMERICAN JOURNAL OF BOTANY
卷 99, 期 4, 页码 E149-E153出版社
BOTANICAL SOC AMER INC
DOI: 10.3732/ajb.1100445
关键词
expressed sequence tags; Leguminosae; microsatellites; pea; Pisum sativum; transferability
资金
- National Natural Science Foundation of China [31000676]
- Zhejiang Provincial Science and Technology Program [2010C32006]
- Zhejiang Important National Science and Technology Specific Projects [2010C02006, 2009C32094]
- Zhejiang Provincial Natural Science Foundation of China [Y3090660]
Premise of the study: Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers were developed in Pisum sativum for further use in genetic studies and breeding programs. Methods and Results: Forty-one novel EST-SSR primers were developed and characterized for size polymorphism in 32 Pisum sativum individuals from four populations from China. In each population, the number of alleles per locus ranged from one to seven, with observed heterozygosity and expected heterozygosity ranging from 0 to 0.8889 and 0 to 0.8400, respectively. Furthermore, 53.7% of these markers could be transferred to the related species, Vicia faba. Conclusions: The developed markers have potential for application in the study of genetic diversity, germplasm appraisal, and marker-assisted breeding in pea and other legume species.
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