期刊
ALLERGY
卷 65, 期 7, 页码 850-858出版社
WILEY
DOI: 10.1111/j.1398-9995.2009.02286.x
关键词
Bet v 1; birch; PM10; pollen; seasonal
资金
- German Ministry of Environment FKZ [20462296]
P>Background: Proof is lacking that pollen count is representative for allergen exposure, also because allergens were found in nonpollen-bearing fractions of ambient air. Objective: We monitored simultaneously birch pollen and the major birch pollen allergen Bet v 1 in different size fractions of ambient air from 2004 till 2007 in Munich, Germany. Methods: Air was sampled with a ChemVol (R) high-volume cascade impactor equipped with stages for particulate matter (PM)> 10 mu m, 10 mu m > PM > 2.5 mu m, and 2.5 mu m > PM > 0.12 mu m. Allergen was determined with a Bet v 1-specific ELISA. Pollen count was assessed with a Burkard pollen trap. We also measured the development of allergen in pollen during ripening. Results: About 93 +/- 3% of Bet v 1 was found in the PM > 10 mu m fraction, the fraction containing birch pollen. We did not measure any Bet v 1 in 2.5 mu m > PM > 0.12 mu m. Either in Munich no allergen was in this fraction or the allergen was absorbed to diesel soot particles that also deposit in this fraction. Pollen released 115% more Bet v 1 in 2007 than in 2004. Also within 1 year, the release of allergen from the same amount of pollen varied more than 10-fold between different days. This difference was explained by a rapidly increasing expression of Bet v 1 in pollen in the week just before pollination. Depending on the day the pollen is released during ripening, its potency varies. Conclusion: In general, pollen count and allergen in ambient air follow the same temporal trends. However, because a 10-fold difference can exist in allergen potency of birch pollen, symptoms might be difficult to correlate with pollen counts, but perhaps better with allergen exposure.
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