期刊
ALLERGY
卷 65, 期 2, 页码 175-183出版社
WILEY
DOI: 10.1111/j.1398-9995.2009.02181.x
关键词
allergy; caveola; epithelium; lipid raft; pollen; systems biology
资金
- Academy of Finland
- Technology Development Centre (TEKES)
- Sigrid Juselius Foundation
- Helsinki University Central Hospital
- Tampere University
- Finnish Society of Allergology and Immunology
- Finnish Society of Otorhinolaryngology
P>Background: The role of epithelium has recently awakened interest in the studies of type I hypersensitivity. Objective: We analysed the nasal transcriptomics epithelial response to natural birch pollen exposure in a time series manner. Methods: Human nasal epithelial cell swabs were collected from birch pollen allergic patients and healthy controls in winter season. In addition, four specimens at weekly intervals were collected from the same subjects during natural birch pollen exposure in spring and transcriptomic analyses were performed. Results: The nasal epithelium of healthy subjects responded vigorously to allergen exposure. The immune response was a dominating category of this response. Notably, the healthy subjects did not display any clinical symptoms regardless of this response detected by transcriptomic analysis. Concomitantly, the epithelium of allergic subjects responded also, but with a different set of responders. In allergic patients the regulation of dyneins, the molecular motors of intracellular transport dominated. This further supports our previous hypothesis that the birch pollen exposure results in an active uptake of allergen into the epithelium only in allergic subjects but not in healthy controls. Conclusion: We showed that birch pollen allergen causes a defence response in healthy subjects, but not in allergic subjects. Instead, allergic patients actively transport pollen allergen through the epithelium to tissue mast cells. Our study showed that new hypotheses can arise from the application of discovery driven methodologies. To understand complex multifactorial diseases, such as type I hypersensitivity, this kind of hypotheses might be worth further analyses.
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