Targeting FAK scaffold functions inhibits human renal cell carcinoma growth

Human conventional renal cell carcinoma (CCC) remains resistant to current therapies. Focal Adhesion Kinase (FAK) is upregulated in many epithelial tumors and clearly implicated in nearly all facets of cancer. However, only few reports have assessed whether FAK may be associated with renal tumorigenesis. In this study, we investigated the potential role of FAK in the growth of human CCC using a panel of CCC cell lines expressing or not the von Hippel-Lindau (VHL) tumor suppressor gene as well as normal/tumoral renal tissue pairs. FAK was found constitutively expressed in human CCC both in culture cells and freshly harvested tumors obtained from patients. We showed that CCC cell growth was dramatically reduced in FAK-depleted cells or after FAK inhibition with various inhibitors and this effect was obtained through inhibition of cell proliferation and induction of cell apoptosis. Additionally, our results indicated that FAK knockdown decreased CCC cell migration and invasion. More importantly, depletion or pharmacological inhibition of FAK substantially inhibited tumor growth in vivo. Interestingly, investigations of the molecular mechanism revealed loss of FAK phosphorylation during renal tumorigenesis impacting multiple signaling pathways. Taken together, our findings reveal a previously uncharacterized role of FAK in CCC whereby FAK exerts oncogenic properties through a non canonical signaling pathway involving its scaffolding kinase-independent properties. Therefore, targeting the FAK scaffold may represent a promising approach for developing innovative and highly specific therapies in human CCC. What's new? Clear cell renal cell carcinoma (CCC) is notorious for its therapeutic resistance, a problem that continues despite advances in drug development. Improvements in CCC survival may now depend on the development of innovative approaches, such as disruption of the scaffolding activities of focal adhesion kinase (FAK). Here, loss of FAK was found to dramatically reduce CCC cell growth, migration, and invasion and to inhibit tumor growth. Mechanistic analyses indicated that FAK acts through kinase-independent scaffolding properties to promote renal tumorigenesis, rather than through kinase activity, as in other cancers.