期刊
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
卷 38, 期 7, 页码 1847-1864出版社
WILEY-BLACKWELL
DOI: 10.1111/acer.12459
关键词
Alcohol; PI3K; Akt; Oxidative Stress; Contractility; Cardiac
资金
- NCRR NIH HHS [2G12 RR003048, G12 RR003048] Funding Source: Medline
- NIAAA NIH HHS [R15 AA019816, 1 R15AA019816-01A1] Funding Source: Medline
- NIMHD NIH HHS [G12 MD007597] Funding Source: Medline
Background: Clinical manifestations of alcohol abuse on the cardiac muscle include defective contractility with the development of heart failure. Interestingly, low alcohol consumption has been associated with reduced risk of cardiovascular disease. Although several hypotheses have been postulated for alcoholic cardiomyopathy and for the low-dose beneficial cardiovascular effects, the precise mechanisms and mediators remain largely undefined. We hypothesize that modulation of oxidative stress by PI3K/Akt plays a key role in the cardiac functional outcome to acute alcohol exposure. Methods: Thus, acutely exposed rat cardiac tissue and cardiocytes to low (LA: 5 mM), moderate (MA: 25 mM), and high (HA: 100 mM) alcohol were assessed for markers of oxidative stress in the presence and absence of PI3K/Akt activators (IGF-1 0.1 mu M or constitutively active PI3K: Ad.BD110 transfection) or inhibitor (LY294002 1 mu M or Akt-negative construct Ad. Akt(K179M) transfection). Results: Acute LA reduced Akt, superoxide dismutase (SOD-3) and NF kappa B, ERK1, and p38 MAPK gene expression. Acute HA only increased that of SOD-3 and NF kappa B. These effects were generally inhibited by Ad. Akt(K179M) and enhanced with Ad. BD110 transfection. In parallel, LA reduced but HA enhanced Akt activity, which was reversed by IGF-1 and inhibited by Ad. Akt(K179M), respectively. Also, LA reduced caspase 3/7 activity and oxidative stress, while HA increased both. The former was blocked, while the latter effect was enhanced by Ad. Akt(K179M). The reverse was true with PI3K/Akt activation. This translated into reduced viability with HA, with no effect with LA. On the functional level, acute LA improved cardiac output and ejection fraction, mainly through increased stroke volume. This was accompanied with enhanced end-systolic pressure-volume relationship and preload recruitable stroke work. Opposite effect was recorded for HA. LA and HA in vivo functional effects were alleviated by LY and enhanced by IGF-1 treatment. Conclusions: Acute LA and HA seem to oppositely affect cardiac function through modulation of oxidative stress where PI3K/Akt plays a pivotal role.
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