期刊
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
卷 38, 期 5, 页码 1293-1300出版社
WILEY-BLACKWELL
DOI: 10.1111/acer.12362
关键词
Cerebellum; Complex Spike; Climbing Fiber; Purkinje Cell; Synaptic; Plasticity
资金
- NIH grants [WR01-AA014973, K12-GM088021]
BackgroundStudies indicate that exposure to ethanol (EtOH) during fetal development damages cerebellar Purkinje cells (PCs). PC proximal dendrites receive glutamatergic input from climbing fibers (CFs) originating at the inferior olive. CF input produces a characteristic response in PCs known as the complex spike (CS). During the first 2weeks of life in rodents (equivalent to the human third trimester of pregnancy), CF-PC synapses undergo profound refinement. Here, we characterized the impact of EtOH exposure during this period on CF-evoked responses in PCs. MethodsUsing vapor chambers, neonatal rat pups and their mothers were exposed to air or EtOH for 4h/d between postnatal day 2 (P2) and P12 (pup serum EtOH concentration, 0.16g/dl). The function of CF-PC synapses was characterized using patch-clamp electrophysiological techniques in acute slices from the cerebellar vermis. Experiments were performed soon after EtOH withdrawal, when perisomatic CFs are still being eliminated (P15 to P17), and after weaning when CF dendritic translocation is almost complete (P21 to P34). ResultsNeither the baseline characteristics of the CS (Na+ spike amplitude, area, coastline index, and afterhyperpolarization [AHP] amplitude) nor the type-1 metabotropic glutamate receptor (mGluR1)-mediated component of both the CS and AHP were significantly affected by EtOH exposure at P15 to P17 or P21 to P34. The mGluR1-dependent long-term depression (LTD) of CF-evoked excitatory postsynaptic currents was not significantly affected by EtOH exposure at P21 to P34. ConclusionsEtOH exposure during the third trimester equivalent neither affected basal characteristics of the CS nor CF-LTD at rat cerebellar PCs from juvenile rats.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据