期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 77, 期 -, 页码 99-104出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.03.009
关键词
Curcuma kwangsiensis; Levan-type fructan; Immunostimulating activity
资金
- 863 National Project [2012AA021403]
- Key Deployment Project of Chinese Academy of Sciences [KSZDEW-Z-019]
A fructan designated as CKNP with apparent molecular weight of 5.3 kD was isolated from the hot water extract of Curcuma kwangsiensis through a combination of ion-exchange chromatography on DEAE 650 M and gel filtration on Superdex G-200. CKNP was characterized by chemical derivatization as well as HPLC, GC, and GC-MS technologies. Structural studies revealed that CKNP is composed predominately of fructose (96.8%) and a small amount of glucose (3.2%) with a degree of polymerization (DP) of 30-31. It was deduced to be a levan-type fructan containing a backbone composed of (2 -> 6)-linked beta-D-Fruf residues and single beta-D-Fruf residues as side chains branched at the O-1 position along the backbone. Preliminary in vitro bioactive tests on RAW 264.7 murine macrophage cells revealed that the levan-type fructan from C. kwangsiensis shows significant immunostimulating activity based on its ability to stimulate macrophage proliferation and enhance phagocytosis. (C) 2015 Elsevier B.V. All rights reserved.
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