期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 73, 期 -, 页码 31-38出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2014.11.001
关键词
Hymenaea courbaril; Polysaccharide characterization; Polysaccharide structure; Viscoelastic fluid; Xyloglucan
资金
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- CNPq
- Centro de Tecnologias Estrategicas do Nordeste (CETENE)
- Coordenagao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacaso para a Ciencia e a Tecnologia (FCT) Strategic Project [PEst-OE/EQB/LA0023/2013]
- Project BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes [NORTE-07-0124-FEDER-000028]
- Programa Operacional Regional do Norte [ON.2-O]
- QREN
- FEDER (Portugal)
Hymenaea courbarilvar courbaril seed xyloglucan was efficiently extracted with 0.1 M NaCl, followed by ethanol precipitation (yield = 72 +/- 5% w/w). Its amorphous structure was identified by the pattern of X-ray diffraction. The monosaccharide composition was determined by GC/MS analysis of the alditol acetates and showed the occurrence of glucose:xylose:galactose:arabinose (40:34:20:6). One-(1D) and two-dimensional-(2D) NMR spectra confirmed a central backbone composed by 4-linked beta-glucose units partially branched at position 6 with non-reducing terminal units of a-xylose or beta-galactose-(1 -> 2)-alpha-xylose disaccharides. The xyloglucan solution was evaluated by dynamic light scattering and presents a polydisperse and practically neutral profile, and at 0.5 and 1.0% (w/v) the solutions behave as a viscoelastic fluid. The polysaccharide did not show significant antibacterial or hemolytic activities. Overall our results indicate that xyloglucan from H. courbaril is a promising polysaccharide for food and pharmaceutical industries. (C) 2014 Elsevier B.V. All rights reserved.
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