Evaluation of anti-inflammatory activity of probiotic on carrageenan-induced paw edema in Wistar rats

Objective: Probiotic strain Lactobacillus sporogenes and Bifidobacteria bifidum were used to assess the anti-inflammatory properties in Carrageenan induced acute inflammatory model. Methods: Non-encapsulated and encapsulated Probiotic strain of Bifidobacteria bifidum and Lactobacillus sporogenes was given orally. Diclofenac sodium was used as standard drug at a concentration of 150 mg/kg of body weight. Edema was induced with 1% carrageenan to all the groups except group A after half an hour of the oral treatments. Paw thickness was checked at t=1, 2, 4 and 24 h. Stair climbing score and motility score were assessed at t=24 h. Results: Non-encapsulated and encapsulated Probiotic Bifidobacteria and Lactobacillus showed a statistically significant decrease in paw thickness at P<0.05. The percentage inhibition in paw thickness of non-encapsulated and encapsulated probiotic L sporogenes and B. bifidum is 37 +/- 3% and 43 +/- 2% after 24 h of treatment. They both significantly increased stair climbing and motility score. Conclusion: Probiotic B. bifidum and L sporogenes significantly decreased the inflammatory reactions induced by carrageenan. (C) 2014 Elsevier B.V. All rights reserved.