期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 72, 期 -, 页码 1041-1043出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2014.10.012
关键词
Chitosa; Osteoblast
In the present study, water-soluble chitosan (WSC) was prepared by hydrolysis of chitosan with commercial alpha-amylase, and the effect of WSC on osteoblast function, i.e. alkaline phosphatase activity (ALP), cell viability, mineralization, and reactive oxygen species (ROS) in osteoblastic MC3T3-E1 cells was investigated. Osteoblastic MC3T3-E1 cells were cultured in various concentrations of WSC solutions (1-5 mg/mL) for designated time and then the ALP, cell viability, mineralization, and ROS in the cells were evaluated. Treatments of osteoblastic with WSC caused a significant increase in ALP, cell viability, and mineralization in osteoblastic MC3T3-E1 cells. Moreover, treatments of osteoblastic with WSC decreased ROS level in osteoblastic MC3T3-E1 cells. The results demonstrate that the WSC may reduce or prevent osteoblasts degeneration through antioxidant activity. (C) 2014 Elsevier B.V. All rights reserved.
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