期刊
AIDS
卷 26, 期 15, 页码 1869-1878出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/QAD.0b013e32835861b0
关键词
AIDS; elite controllers; HIV-1; human leukocyte antigen; killer inhibitory receptor; natural killer cells; T cells
资金
- National Institutes of Health [R21 AI078870, NIDA R01 DA028775, R01 AI073219, RO1 AI065279, P30 CA10815]
- Philadelphia Foundation
- Pennsylvania Commonwealth Universal Research Enhancement Program
- Centers for AIDS Research at UCSF [PO AI27763]
- CFAR Network of Integrated Systems [R24 AI067039]
- UCSF CTSI [UL1 RR024131]
- NIAID [RO1 AI087145, K24AI069994, AI 76174]
- amfAR
- Ragon Institute
- National Cancer Institute, National Institutes of Health [HHSN261200800001E]
- NIH, National Cancer Institute, Center for Cancer Research
Objective: Both protective T-cell genotypes and natural killer (NK) cell genotypes have been associated with delayed progression to AIDS and shown to be co-inherited in HIV-1-infected individuals who limit viral replication in absence of antiretroviral therapy ('controllers'). However, a comparative analysis of the genotype and function of the innate and adaptive immune compartments in HIV-1-infected controller individuals has been understudied to date. Design: Here, we simultaneously tested NK and T-cell function in controllers to investigate the mechanism(s) thatmight account for host immune control over viral replication. Methods: We measured CD8 T-cell responses against HIV-1 utilizing overlapping 15-mer peptides spanning the HIV-1 consensus clade B Gag protein and tested NK cell degranulation and cytokine secretion against tumor target cells following interferon-alpha (IFN alpha) stimulation. Results: Among a cohort of 37 controllers, the presence of protective major histocompatibility complex class I human leukocyte antigen (HLA) alleles (such as HLA-B*57) was not correlated with HIV-specific CD8 responses. In contrast, the inheritance of a protective killer inhibitory receptor KIR3DL1*h/*y receptor genotype along with the corresponding HLA-Bw4*80I ligand was associated with significantly heightened target cell-induced NK degranulation and cytokine secretion following IFN alpha stimulation (P = 0.0201, n = 13). Interestingly, we observed a significant inverse association between the IFN alpha stimulated NK response to K562 cells and the HIV-specific CD8 T-cell response to Gag among elite controllers (rho = -0.8321, P = 0.0010, n = 12). Conclusion: Together, these results suggest that heightened NK responses can be evidenced independently of HIV-specific T-cell responses in HIV-1-infected elite controllers. (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins
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