期刊
AIDS
卷 24, 期 7, 页码 947-957出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/QAD.0b013e328337b144
关键词
AIDS; CD107a; CD154; CD4; gag; HIV; long-term nonprogressor; lymphocyte degranulation; specific response
资金
- Istituto Superiore di Sanita, Rome, Italy [40G.62]
Background: The activity of virus-specific T lymphocytes, among which those capable of a polyfunctional response against the viral protein gag, is crucial to control HIV infection. Objective: The objective of this study is to investigate the polyfunctionality of gag-specific T cells in different phases of HIV infection, analyzing markers related to T-helper cell 1 (Th1) and degranulation/cytotoxicity, and the production of Th1 cytokines in peripheral blood lymphocytes from patients experiencing an acute primary infection, long-term nonprogressors, patients naive for antiretroviral drugs, and patients taking HAART. Materials and methods: Cells were stimulated with a pool of gag-derived peptides or with a superantigen (staphylococcal enterotoxin B). Using eight-color polychromatic flow cytometry, we analyzed the expression of interleukin-2, interferon-gamma, CD154, and CD107a by CD4(+) and CD8(+) T cells. Results: The main finding was that in all HIV-positive patients, about half gag-specific CD4(+) T cells were CD107a(+), that is, able to degranulate. CD4(+)CD154(+) cells unable to produce Th1 cytokines were the second most represented population. Truly polyfunctional CD4(+) T cells were very rare and present only in a few long-term nonprogressors. Superantigen stimulation showed that CD4(+) T lymphocytes from all patients displayed a typical Th response, including interleukin-2 and interferon-gamma production, lacking CD107a expression. Conclusion: In all the aforementioned phases of HIV infection, the large majority of gag-specific CD4(+) T lymphocytes cannot be identified by the sole expression of interleukin-2 and interferon-gamma, which is early impaired. Degranulation and helper functions other than Th1 cytokine production are the predominant features of HIV-specific CD4(+) lymphocytes. (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins
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