期刊
AICHE JOURNAL
卷 55, 期 3, 页码 797-806出版社
WILEY
DOI: 10.1002/aic.11700
关键词
ultrasound; soapstock; gossypol; gossypol acetic acid; anticancer
资金
- National Natural Science Foundations of China [30570494, 30772658, 30710403089]
- USA Department of Defense Prostate Cancer Research Program [W81XWII-04-1-0215, W81XWH-06-1-0010]
- Shaanxi Provincial Department of Education Science Research Program [08JK218]
- Shaanxi Institute of Education Science Research Program in China [09KJ07]
To investigate the extracted process of gossypol acetic acid (G-AA) from cottonseed soapstock and explore the improvement of its yield and purity, a novel ultrasound-assisted extraction and crystallization method was introduced to this process. Under the optimized conditions, preliminary G-AA with the yield of 1300 mg and the purity of 95.9% could be obtained from 100 g of fresh soapstock by ultrasound-assisted extraction. In addition, UV, IR, and NMR spectrum further confirmed the detailed chemical structure of G-AA. Assay of inhibiting human prostate tumor cell line PC-3 and human breast cancer cell line MDA-MB-231 revealed its biological activity, the values of IC50 are 9.096 mu mol/L and 14.37 mu mol/L respectively. In comparison with the conventional solvent extraction, this novel process increases the content of G-AA over 90%, reduces the little of crystallization by 75%, and retains the anticancer activity of gossypol. (c) 2009 American Institute of Chemical Engineers AIChE J, 55: 797-806, 2009
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