期刊
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
卷 38, 期 4, 页码 210-223出版社
SAGE PUBLICATIONS LTD
DOI: 10.5301/ijao.5000404
关键词
Cartilage; Regenerative medicine; Biocompatible materials; Tissue scaffolds; Experimental animal models
资金
- Spanish Ministry of Science and Innovation [MAT2010-21611-C03-00]
- FEDER
- Conselleria de Educacion (Generalitat Valenciana, Spain) [PROMETEO/2011/084]
- CIBER-BBN en Bioingenieria, Biomateriales y Nanomedicina
- Generalitat Valenciana [ACOMP/2012/075]
- VI National RDi Plan
- Iniciativa Ingenio
- Consolider Program
- CIBER Actions
- Instituto de Salud Carlos III
- European Regional Development Fund
Purpose: Tissue engineering techniques were used to study cartilage repair over a 12-month period in a rabbit model. Methods: A full-depth chondral defect along with subchondral bone injury were originated in the knee joint, where a biostable porous scaffold was implanted, synthesized of poly(ethyl acrylate-co-hydroxyethyl acrylate) copolymer. Morphological evolution of cartilage repair was studied 1 and 2 weeks, and 1, 3, and 12 months after implantation by histological techniques. The 3-month group was chosen to compare cartilage repair to an additional group where scaffolds were preseeded with allogeneic chondrocytes before implantation, and also to controls, who underwent the same surgery procedure, with no scaffold implantation. Results: Neotissue growth was first observed in the deepest scaffold pores 1 week after implantation, which spread thereafter; 3 months later scaffold pores were filled mostly with cartilaginous tissue in superficial and middle zones, and with bone tissue adjacent to subchondral bone. Simultaneously, native chondrocytes at the edges of the defect started to proliferate 1 week after implantation; within a month those edges had grown centripetally and seemed to embed the scaffold, and after 3 months, hyaline-like cartilage was observed on the condylar surface. Preseeded scaffolds slightly improved tissue growth, although the quality of repair tissue was similar to non-preseeded scaffolds. Controls showed that fibrous cartilage was mainly filling the repair area 3 months after surgery. In the 12-month group, articular cartilage resembled the untreated surface. Conclusions: Scaffolds guided cartilaginous tissue growth in vivo, suggesting their importance in stress transmission to the cells for cartilage repair.
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