期刊
APPLIED OPTICS
卷 48, 期 22, 页码 4437-4448出版社
OPTICAL SOC AMER
DOI: 10.1364/AO.48.004437
关键词
-
类别
资金
- P2R Franco-Israeli Collaborative Research Program
- French National Research Agency (ANR)
- CORDI-S
- CNRS
We propose an alternate minimization algorithm for estimating the point-spread function (PSF) of a confocal laser scanning microscope and the specimen fluorescence distribution. A three-dimensional separable Gaussian model is used to restrict the PSF solution space and a constraint on the specimen is used so as to favor the stabilization and convergence of the algorithm. The results obtained from the simulation show that the PSF can be estimated to a high degree of accuracy, and those on real data show better deconvolution as compared to a full theoretical PSF model. (C) 2009 Optical Society of America
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据