期刊
AGING CELL
卷 12, 期 1, 页码 76-84出版社
WILEY
DOI: 10.1111/acel.12026
关键词
astrocytes; DHA; glutamate; hippocampus; neurotransmission; polyunsaturated fatty acids
资金
- French Research Agency (ANR) [ANR-09-ALIA-006-01]
Epidemiological data suggest that a poor ?3 status favoured by the low ?3/?6 polyunsaturated fatty acids ratio in western diets contributes to cognitive decline in the elderly, but mechanistic evidence is lacking. We therefore explored the impact of ?3 deficiency on the evolution of glutamatergic transmission in the CA1 of the hippocampus during aging by comparing 4 groups of rats aged 622 months fed ?3-deficient or ?3/?6-balanced diets from conception to sacrifice: Young ?3 Balanced (YB) or Deficient (YD), Old ?3 Balanced (OB) or Deficient (OD) rats. ?3 Deficiency induced a 65% decrease in the amount of docosahexaenoic acid (DHA, the main ?3 in cell membranes) in brain phospholipids, but had no impact on glutamatergic transmission and astroglial function in young rats. Aging induced a 10% decrease in brain DHA, a 35% reduction of synaptic efficacy (fEPSP/PFV) due to decreased presynaptic glutamate release and a 30% decrease in the astroglial glutamate uptake associated with a marked astrogliosis (+100% GFAP). The ?3 deficiency further decreased these hallmarks of aging (OD vs. OB rats: -35% fEPSP/PFV P < 0.05, -15% astroglial glutamate uptake P < 0.001, +30% GFAP P < 0.01). This cannot be attributed to aggravation of the brain DHA deficit because the brains of OD rats had more DHA than those of YD rats. Thus, ?3 deficiency worsens the age-induced degradation of glutamatergic transmission and its associated astroglial regulation in the hippocampus.
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