期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 45, 期 8, 页码 517-526出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2015.02.010
关键词
Echinococcus multilocularis; p53; DNA damage; Apoptosis; Oxidative stress
类别
资金
- Fundamental Research Funds for the Central Universities of People's Republic of China [2010121091, 2012121044]
- National Natural Science Foundation of China [81000740, 81171596, 81201305, 81471970]
Larvae of the fox tapeworm, Echinococcus multilocularis, cause alveolar echinococcosis, which is considered to be the most lethal helminthic infection in humans. Since it develops in host organs, the parasite must have evolved a stress defense system to cope with various genotoxic and cellular stresses that may cause DNA damage and genomic instability. Tumor suppressor p53, well known as the guardian of the genome, plays a vital role in response to many types of stress and damage. In the present study, we describe the characterisation of Emp53 from E. multilocularis and demonstrate that it is a structural and functional homologue of mammalian tumor suppressor p53. We show that Emp53 binds specifically to oligonucleotides containing conventional p53 binding sites, indicating that it exhibits a function as a DNA binding transcription factor. Inhibition of Emp53 function can suppress UV irradiation-induced apoptosis in the E. multilocularis metacestode, indicating an important role of Emp53 in the induction of apoptosis following DNA damage. We also reveal that Emp53 plays important roles in resistance to oxidative stress and regulation of oxidative stress-induced apoptosis. Our results suggest that, similar to its human counterpart, Emp53 plays a central role in the network of DNA damage responses and apoptosis in E. multilocularis. These results may help in exploring stress defense mechanisms of parasitic helminths and may provide useful information for the development of new interventions and therapeutic drugs for the control of alveolar echinococcosis. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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