期刊
AEROBIOLOGIA
卷 30, 期 2, 页码 205-209出版社
SPRINGER
DOI: 10.1007/s10453-013-9315-1
关键词
Mycoplasma hyopneumoniae; Air sample; PCR; Swine farm
资金
- National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2011-0021670]
- Rural Development Administration
- ministry of Knowledge Economy (MKE)
- Korea Institute for Advancement of Technology 15 (KIAT) through the Center ER Cooperation project
We aimed to detect Mycoplasma hyopneumoniae from the air of selected farms through air filtration-based air sampling using polymerase chain reaction (PCR). Air samples were collected at different locations inside and outside of pig farm rooms on polyethersulfone membrane (0.22 mu m), and the presence of M. hyopneumoniae DNA was analyzed by PCR. Furthermore, nasal swab and blood samples were collected from 336 pigs in the same air sampled rooms and were analyzed by PCR and IDEXX ELISA, respectively. The suitability of the air sampling method was validated with analysis of an artificially induced aerosolized avirulent M. hyopneumoniae in an enclosed box showing PCR-positive results. M. hyopneumoniae was detected from air sample of pig farm rooms using PCR. Although the probability of an airborne M. hyopneumoniae causing an infection is not yet confirmed, air sampling PCR results could serve as a tool to assess the spread of M. hyopneumoniae by bioaerosols and the infection dynamics in a herd and between herds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据