Leonurine ameliorates kidney fibrosis via suppressing TGF-β and NF-κB signaling pathway in UUO mice

Fibrosis is one of the characteristic features of chronic kidney disease (CKD). Inflammatory reactions and oxidative stress are implicated in the pathogenesis of fibrosis of CKD. Leonurine (LEO) is one of the active compounds from Herba leonuri. In this study, we further evaluated its renoprotective effect in a mouse unilateral urethral obstruction (UUO), featuring the renal tubulointerstitial fibrosis and inflammation. In this model, pretreat of LEO before ureteral obstruction abolished the expression of fibronectin, suppressed the expression of alpha-SMA and type I/III collagen and down-regulated vimentin. LEO also modified the cytokine expression of TGF-beta, TNF-alpha, IL-6 and IL-1 beta and suppressed the phosphotylation of Smad3. Moreover, LEO blocked phosphotylation of NF-kappa B, and inactivated the signaling pathways associated with the progression of kidney inflammatory response. Our data support that LEO is a candidate renoprotective compound for renal fibrosis through targeting the TGF-beta/Smad3 and NF-kappa B pathway. (C) 2015 Elsevier B.V. All rights reserved.