期刊
ADVANCES IN SPACE RESEARCH
卷 53, 期 1, 页码 18-29出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.asr.2013.08.001
关键词
Bacillus cereus; Space flight; Phenotype; Next-generation; RNA-seq; iTRAQ
类别
资金
- National Basic Research Program of China (973 program) [2014CB744400]
- Key Pre-Research Foundation of Military Equipment of China [9140A26040312JB10078]
- Key Program of Medical Research in the Military '12th 5-year Plan, China [BWS12J046]
- Ministry of Education of PR China [(190)H0511009]
The environment in space could affect microorganisms by changing a variety of features, including proliferation rate, cell physiology, cell metabolism, biofilm production, virulence, and drug resistance. However, the relevant mechanisms remain unclear. To explore the effect of a space environment on Bacillus cereus, a strain of B. cereus was sent to space for 398 h by ShenZhou VIII from November 1, 2011 to November 17, 2011. A ground simulation with similar temperature conditions was simultaneously performed as a control. After the flight, the flight and control strains were further analyzed using phenotypic, genomic, transcriptomic and proteomic techniques to explore the divergence of B. cereus in a space environment. The flight strains exhibited a significantly slower growth rate, a significantly higher amikacin resistance level, and changes in metabolism relative to the ground control strain. After the space flight, three polymorphic loci were found in the flight strains LCT-BC25 and LCT-BC235. A combined transcriptome and proteome analysis was performed, and this analysis revealed that the flight strains had changes in genes/proteins relevant to metabolism. In addition, certain genes/proteins that are relevant to structural function, gene expression modification and translation, and virulence were also altered. Our study represents the first documented analysis of the phenotypic, genomic, transcriptomic, and proteomic changes that occur in B. cereus during space flight, and our results could be beneficial to the field of space microbiology. (C) 2013 COSPAR. Published by Elsevier Ltd. All rights reserved.
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