期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 27, 期 1, 页码 156-163出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2015.05.013
关键词
Allyl methyl disulfide; IL-8; IP-10; TNF-alpha; NF-kappa B
资金
- National Major Science and Technology Project-Prevention and Treatment of AIDS, Viral Hepatitis, and Other Major Infectious Diseases [2013ZX10005004]
Garlic and its active constituents have shown versatile medicinal activities in the prevention and treatment of various disorders. Allyl methyl disulfide (AMDS) was identified as one of the major bioactive components in an effective inhalation fork remedy using fresh garlic paste in our previous study. In this work, we investigated the immunological properties of AMDS to elucidate the underlying mechanisms of the fork inhalation treatment using fresh garlic. The inhibition effect of AMDS on TNF-alpha-induced IL-8 and IP-10 production in intestinal epithelial cell lines HT-29 and Caco-2 was first evaluated. Pretreatment of the cells with AMDS attenuated IL-8 and IP-10 secretion induced by TNF-alpha in a dose-dependent manner in the non-cytotoxic concentration range of 20 to 150 mu M. Mechanistic studies revealed that AMDS suppressed the accumulation of IL-8 mRNA and inhibited I kappa B alpha degradation and NF-kappa B p65 translocation into the nucleus at both the transcriptional and translational levels, suggesting that the attenuation effort of AMDS on cytokine IL-8 secretion might at least be partially related to the NF-kappa B signaling pathway. These results suggest that AMDS may be a promising phytochemical agent in the treatment of immunological disorders, such as ulcerative colitis, Crohn's disease, intestinal inflammatory diseases and others. In addition, the mechanistic study data indicated that immune modulation could be one of the therapeutic mechanisms of the effective fork treatment containing AMDS as one of the major components. (C) 2015 Elsevier EV. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据