Effects of selenizing angelica polysaccharide and selenizing garlic polysaccharide on immune function of murine peritoneal macrophage

The effects of two selenizing polysaccharides (sCAP(2) and sGPS(6)) on immune function of murine peritoneal macrophages taking two non-selenizing polysaccharides (CAP and GPS) and modifier Na2SeO3 as control. In vitro test, the changes of selenizing polysaccharides, non-selenizing polysaccharides and Na2SeO3 on murine macrophages function were evaluated by phagocytosis and nitric oxide (NO) secretion tests. In vivo test, the mice were injected respectively with 0.2, 0.4 and 0.6 mg of sCAP2, sGPS6, CAP and GPS, or Na2SeO3 80 Kg or normal saline 0.4 mL. The peritoneal macrophages were collected and cultured to determine the contents of TNF-alpha, IL-6 and IL-10 in supernatants by enzyme-linked immunosorbent assay. The results showed that sCAP(2) and sGPS6 could significantly promote the-phagocytosis and secretion of NO and three cytokines of macrophages in comparison with CAP and GPS. sCAP(2) possessed the strongest activity. This indicates that selenylation modification can further improve the immune-enhancing activity of polysaccharide, and sCAP(2) could be as a new immunopotentiator. (C) 2015 Elsevier B.V. All rights reserved.