期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 27, 期 1, 页码 32-37出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2015.04.024
关键词
Chemokines; ICAM-1; MAPK; NF-kappa B; Phloretin
资金
- Chang Gung Memorial Hospital [CMRPF1D0101, CMRPF1B0131-3]
Previous studies found that phloretin had anti-oxidant, anti-inflammatory, and anti-tumor properties. In this study, we investigated whether phloretin could suppress the production of the intercellular adhesion molecule (ICAM)-1 and chemokines through downregulation of the nuclear transcription factor kappa-B (NF-kappa B) and mitogen-activated protein kinase (MAPK) signaling pathways in TNF-alpha-stimulated HaCaT human keratinocytes. HaCaT cells were treated with phloretin and then the cells were stimulated by TNF-alpha. Phloretin treatment decreased the production of IL-6, IL-8, CCL5, MDC, and TARC. Phloretin decreased ICAM-1 protein and mRNA expression, and also suppressed the adhesion of monocyte THP-1 cells to inflammatory HaCaT cells. Phloretin inhibited NF-kappa B translocation into the nucleus and also suppressed the phosphorylation of Akt and MAPK signal. In addition, phloretin increased heme oxygenase-1 production in a concentration-dependent manner. These results demonstrated that phloretin has anti-inflammatory effects to inhibit chemokines and ICAM-1 expressions through suppression of the NF-kappa B and MAPK pathways in human keratinocytes. (C) 2015 Elsevier B.V. All rights reserved.
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